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Novel drought-inducible Cys2/His2-type zinc finger protein STF-2 from soybean (Glycine max) enhances drought tolerance in transgenic plants
Abstract
The C2H2-type (Cys2/His2-type) zinc finger proteins are transcription factors which play important roles in growth, development and tolerance ability to adverse stresses. We characterized STF-2 from Glycine max to determine its molecular structure and biological function. The results showed that STF-2 was a novel plant C2H2-type zinc finger protein gene and encoded a 26.7-kDa protein comprising 249 amino acids. STF-2 is strongly expressed in flowers and leaves but only weakly expressed in stems and roots. We constructed a plant expression vector with STF-2 gene and transformed it into tobacco. We found that overexpression of STF-2 enhanced drought tolerance in transgenic tobacco. Our results indicate the STF-2 plays an important role in soybean response to drought stress; furthermore, plant resistance to adversity can be improved by exogenous expression of this gene.

