PJB-2018-1568
The effects of AgNO3 and 2iP (N6-(2-Isopentenyl) adenine) on different stages of somatic embryogenesis in date palm (Phoenix dactylifera L. cv. Medjool)
Behnaz Abdolvand, Reza Zarghami and Ali Salari
Abstract
This study is aimed at investigating the effects of silver nitrate (AgNO3) during various stages of somatic embryogenesis in Medjool palm. Silver nitrate is a strong inhibitor of ethylene, which by reducing the negative effects of ethylene, confers growth and development on tissue culture plants in laboratory. In the present study, the effects of different concentrations of AgNO3 and 2iP on embryogenic callus production, the number of embryos, the length of embryos and the germination of somatic embryos were scrutinized. Moreover, the length of the plantlet, rooting percentage, length of root, and the quality of plantlets were examined under effect of different concentrations of AgNO3. The media supplemented with 4 mg/l of AgNO3 induced the highest number of embryogenic callus and the greatest number of embryos. While, the media contained 0.5 mg/l 2iP resulted in the highest length of embryos. Furthermore, a media including 8 mg/l of silver nitrate has a very positive effect on the germination of somatic embryos. In order to prolonging the plantlets and root length, AgNO3 is an invigorating treatment to be utilized, but it did not have a positive effect on rooting of the plantlets. The genetic stability of tissue culture plants derived from somatic embryogenesis, examined and screened by 16 primer pairs of SSR marker. As a result, it had been revealed that somatic embryogenesis method and the protocol used in this study were the suitable and safe method for proliferation of Medjool cultivar in date palm, as no differences were observed among In vitro grown plants and the mother plant.
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