PJB-2018-1598
Transcriptional changes of BFRUCT3, NHX1, OMT and PEAMT genes in root of barley (Hordeum vulgare L.) under salinity stress
Roghayeh Ghorbani, Seyyed Abolghasem Mohammadi, Mahmood Toorchi and Sara Ghafarian
Abstract
The expression changes of BFRUCT3, OMT, NHX1, PEAMT genes in root of barley genotypes; Clipper (sensitive), Sahara3771 (tolerant) and an Iranian advanced line (tolerant) were evaluated under 0, 100 and 200 mM NaCl at 24 hours, 3 days and 3 weeks after salt treatment. Expression of the genes was analyzed using Real-Time PCR Based on 2-ΔΔCT data obtaining from the comparison of different salinity treatments. Analysis of transcript level of BFRUCT3 gene revealed up-regulation of this gene in the salt tolerant genotypes with prolonging of salt treatment duration and increase of salinity level from 100 to 200 mM NaCl. Whereas, OMT gene showed up-regulation in all the three genotypes with increase of salinity level at the earlier stage of salt treatment. With increase of salt level to 200 mM NaCl, PEAMT gene showed up-regulation in the advanced line at the early time points (24h and 3 days) and in the Sahara3771 at the late time point (3 weeks) but in Clipper expression of this gene was down-regulated.
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