PJB-2018-1602
Effects of sucrose and growth regulators on the microtuberization of CIP potato (Solanum tuberosum) germplasm
Shahid Ali, Naeem Khan, Faisal Nouroz, Shazia Erum and Wajid Nasim
Abstract
The present experiment was aimed to study the effect of sucrose and growth regulators (kinetin and 6- benzylaminopurine) on potato microtuber formation. For this purpose the Murashige and Skoog (MS) media, supplemented with different levels of sucrose concentration and growth regulators had been used. The result showed that MS medium with double level (60g) of sucrose without any growth regulator had significantly enhanced the tuber formation but T1 treatment (i.e.100g of sucrose) had no significant effect on tuber formation. It was revealed that sucrose had positive impacts on the microtuberization up to some extent but at very high concentration reduced the microtuber formation. The kinetin and 6-benzylaminopurine (BAP) also enhanced the microtuber formation. Early tuber initiation was observed in kinetin treated plants as compared to other treatments and BAP treated plants showed increase in weight of microtubers. The study also revealed that increase in density of plantlets had negatively affected the number of microtuber formation but the kinetin and BAP had positive impacts to enhance the microtuber formation. Dark condition provides better response as compared to light condition. The two explants source (nodal and sprout explants) the nodal explants showed best results as compared sprout explants as tuber initiation and weight of microtuber
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