PJB-2018-1604
Brassica oleracea L. var. Italica Plenck and Cassia absus L. extracts reduce oxidative stress, alloxan induced hyperglycemia and indices of diabetic complications
Sidra Khalid, Shahzad Hussain, Hussain Ali, Muhammad Khalid Tipu, Humaira Fatima, Madiha Ahmed and Tofeeq Ur-Rehman
Abstract
The nature’s endowment of medicinal plants in successful management of diabetes necessitates their further exploration. Therefore, the present study was designed to comprehend ameliorating role of Brassica oleracea var. italic (BO) and Cassia absus (CA) in oxidative stress, hyperglycemia and indices of diabetic complications. Among all the extracts of BO and CA, aqueous extract was the most proficient in terms of extract recovery (9.0 and 10.2%) and DPPH radical scavenging efficiency (IC50 = 11.90 ± 1.70 and 8.26 ± 1.20 μg/ml) respectively. Maximum phenolic content [BO = 184.0 ± 0.17 and CA = 406.7 ± 0.08 μg gallic acid equivalent/mg extract (E)], flavonoid content (BO = 160.9 ± 0.1 and CA = 361.9 ± 0.09 μg quercetin equivalent/mg E) and total antioxidant capacity (BO = 223.7 ± 0.20 and CA = 257.2 ± 3.30 μg ascorbic acid equivalent/mg E) was recorded in their ethanol extract. Highest reducing power potential was quantified in BO ethanol and CA aqueous extractsas 427.9 ± 0.10 and 480.0 ± 2.10μg ascorbic acid equivalent/mg E respectively. Brine shrimp assay expounded petroleum ether extract of BO and CA to have some cytotoxicity (LC50 = 200± 2.3 and 86.6 ± 3.1 µg/ml respectively). In vivo studies established their aqueous extract as proficient in reducing the serum glucose (BO = 142.3 ± 7.10 and CA = 161.5 ± 4.40 mg/dl at day 21) as well as blood cholesterol, ALT and urea levels. Findings of the present study prospects BO and CA a useful treatment of diabetes and its escorting complications.
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