PJB-2018-1610
In vitro anti-pseudomonal potential of Juglans regia and Otostegia limbata leaves extract against planktonic and biofilm form of Pseudomonas aeruginosa
Imran Khan, Uzma Khan, Kamran Khan, Muhammad Asif Nawaz, Nazir Ahmad Khan and Fayaz Ali
Abstract
In the present study the anti-pseudomonal potential of crude methanolic extracts, hexane, ethyl acetate and water fractions of the leaves of Juglans regia L. and Otostegia limbata (Benth.) Boiss.against planktonic and biofilm form of clinical strains (P1, P2 and P3 strains) of Pseudomonas aeruginosa (P. aeruginosa) were evaluated. Agar well diffusion and minimum inhibitory concentration (MIC) assays were used against planktonic, whereas pellicle inhibition and static biofilm inhibition assays were performed against biofilm form of P. aeruginosa. In well diffusion assay, the crude methanolic extract of J. regia showed good bacterial inhibition than O. limbata. The J. regiacrude methanol extract had significant (+; complete breakage of pellicle layer), good (++; partial breakage of pellicle layer) and moderate (+++; uniformthin layer of pellicle formation) pellicle inhibition activity, while O. limbata had moderate (+++; uniformthin layer of pellicle formation) to weak (++++; loose thick layer pellicle formation) pellicle inhibition effect. In MIC assays, hexane and water fractions of J. regia had high (86 vs. 77%) antibacterial activity, while crude methanolic extract of O. limbata showed 51% inhibition against the most resistant P3 strain at 1000 µg/ml concentration. In static antibiofilm assay, hexane fraction of J. regia had high (63%) inhibition compared to crude methanolic extract of O. limbata(31%) against P3 strain. The present study highlights that J. regia extracts possesses high anti-pseudomonal properties as compared to O. limbata.
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