PJB-2018-381
Protective effect of essential oils of Ocimum basilicum, Galium odoratum, and Cymbopogon citratus against oxidative DNA damage in cultured human lymphocyte cells
Omar F. Khabour, Karem H. Alzoubi, Salwa F. M. Hassanein, Hanin Makhlouf and Farah Alhashimi
Abstract
The potential of essential oils as antioxidants is gaining interest by researchers and medicinal industry. In this study, we investigated the protective effect of essential oils obtained from Ocimum basilicum, Galium odoratum, and Cymbopogon citratus against oxidative DNA damage in cultured human lymphocytes. DNA damage was assessed using 8-hydroxy deoxyguanosine assay (8-OHdG). The oil of G. odoratum showed significant decreases of 8-OHdG biomarker in cultured cells at all examined concentrations (0.1, 1 and 2 uL/mL) with magnitudes that range between 31-39% (p<0.01). With respect to C. citratus oil, a significant decrease of 8-OHdG was observed at 1 and 2 µL/mL concentrations with magnitudes of 34% and 44% respectively (p<0.05). Similarly, O. basilicum significantly decreased 8-OHdG at 1µL/mL and 2 µL/mL concentrations (p<0.005) with magnitude of decreases of about 62% at both concentrations. In conclusion, potent antioxidative DNA damage activity of examined essential oils was observed in cultured human lymphocytes.
To Cite this article:
Khabour, O.F., K.H. Alzoubi, S.F.M. Hassanein, H. Makhlouf and F. Alhashimi. 2023. Protective effect of essential oils of Ocimum basilicum, Galium odoratum, and Cymbopogon citratus against oxidative DNA damage in cultured human lymphocyte cells. Pak. J. Bot., 55(1): DOI: http://dx.doi.org/10.30848/PJB2023-1(4)
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