PJB-2004-44
DIVERSITY OF BIPOLARIS SOROKINIANA ISOLATES FROM WHEAT GROWING AREAS UNDER RICE-WHEAT CROPPING SYSTEM IN PUNJAB, PAKISTAN
SHAZIA IRAM AND IFTIKHAR AHMAD
Abstract
A survey was conducted for the assessment of foliar blight of wheat in main rice-wheat cropping areas of the Punjab province, Pakistan. The foliar fungus Bipolaris sorokiniana was isolated from leaf samples. Isolates of B. sorokiniana were classified according to their aggressive behavior based on disease severity scale. B. sorokiniana isolates showed foliar blight symptoms on wheat but not on rice. Random Amplified Polymorphism DNA (RAPD) was used to study the genetic variation within the populations of the fungus. A correlation study was carried out with the help of five primers viz., P1 (5´-AGGAGGACCC-3´), P2 (5´-ACGAGGGACT-3´), PE7 (5´-AGATGCAGCC-3´), P14 (5´-CCACAGCACG-3´) and PE20 (5´-AACGGTGACC-3´). A tree was constructed based on the pattern of bands which highlighted the correlation between morphological, aggressiveness and genetic variations of B. sorokiniana.
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