PJB-2018-524
Identification and mapping of an early leaf senescence trait, es2 mutant, in rice
Sittipun Sinumporn, Yuyu Chen, Peipei Zhang, Yingxin Zhang, Liaqat Shah, Yongrun Cao, Ning Yu, Ping Yu, Weixun Wu, Liyong Cao and Qunen Liu
Abstract
Leaf senescence is the final stage of leaf development. Early senescence has negative impacts on crop productivity, especially in rice. In this study, early leaf senescence on chromosome 2 mutant, designated as es2, was generated by ethyl methanesulfonate (EMS) mutagenesis. Senescence symptom in es2 appeared at the late tillering stage, and was coincidence with elevated level of cell death, sugar content, and facilitated by senescence-associated genes (SAGs) transcription. Further, oxidative stress such as hydrogen peroxide (H2O2), oxidative stress byproducts such as malondialdehyde (MDA), and reactive oxygen species (ROS) contents were elevated in es2 while soluble protein content was decreased. Chloroplast ultrastructure of es2 was filled with larger in size of starch granule and higher in number of osmophilic plastoglobuli, along with looser thylakoid symptom. At the heading stage, es2 exhibited sharply decreasing of net photosynthetic rate (NPR) beginning from 14-28 days after heading. Consistently, es2 photosynthetic rate was dramatically lower than wild type (WT) in flag leaf, the second leaf from top, and the third leaf from top. Using map-based cloning technique, we identified that the target gene was anchored between RM12538 and RM12729 on the short arm of chromosome 2. Finally, this research provides a novel early leaf senescence mutant characterization in rice
To Cite this article:
Sinumporn, S., Y. Chen, P. Zhang, Y. Zhang, L. Shah, Y. Cao, N. Yu, P. Yu, W. Wu, L. Cao and Q. Liu. 2020. Identification and mapping of an early leaf senescence trait, es2 mutant, in rice. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(36)
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