PJB-2018-672
Physiological and anatomical changes in Thai rice landrance (Oryza sativa L.) cv Pakaumpuel after colchicine treatment
Worasitikulya Taratima, Pradub Reanprayoon, Sayam Raso, Mallika Chantarangsee and Pitakpong Maneerattanarungroj
Abstract
Physiological and anatomical characteristics of the Thai rice landrace cv Pakaumpuel were investigated. Dehusked seeds were soaked in various concentrations of colchicine for 24 and 48 h in the dark, prior to washing in distilled water three times before germination. Two weeks’ old seedlings were cultured up to 16 weeks with transfer into bigger pots and physiological and anatomical parameters were recorded. The abaxial epidermis of mature leaves was examined by peeling technique, while leaf blade anatomy was investigated by transverse free hand section using 2% (w/v) Safranin as staining agent. Thirty-one anatomical characteristics were recorded. Results showed that colchicine treatment increased all growth parameters with the exception of tiller number. Eighteen anatomical characteristics showed significant differences after treatments including guard cell length, midrib thickness, and vertical and horizontal length of midrib and lamina vascular bundles. Effects of colchicine on growth and anatomical characteristics provide confirmatory evidence for polyploidy. This is the first report on Thai rice landrace anatomy. Basic data may be useful for product improvements in other rice cultivations.
To Cite this article:
Taratima, W., P. Reanprayoon, S. Raso, M. Chantarangsee and P. Maneerattanarungroj. 2020. Physiological and anatomical changes in Thai rice landrance (Oryza sativa L.) cv Pakaumpuel after colchicine treatment. Pak. J. Bot., 52(5): DOI: http://dx.doi.org/10.30848/PJB2020-5(24)
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