PJB-2018-708
Authentication of indigenously developed chilli (Capsicum annuum L.) hybrids through ISSR markers
Tahira Noor, Armghan Shahzad, Nausherwan Nobel Nawab, Ghulam Muhammad Ali and Aasia Ramzan
Abstract
Hybrid technology promises higher yields. For Capsicum annuum L., hybrid production is preferred because the vigorous yield uniformity, tolerance against biotic and abiotic stresses and other excellent horticultural traits. From the breeder’s perspective, development of hybrids permits better control of intellectual property rights including control and protection of parental lines. Nowadays elite chilli hybrids have become popular due to their best performance. At present indigenously developed 10 elite chilli hybrids (Hybrid-1 to Hybrid-10) along with their 6 elite parents/inbred lines were used for molecular study to authenticate the F1 progeny. The DNA based hybrid purity testing was carried out by using 16 inter simple sequence repeat polymerase chain reaction (ISSR-PCR) markers. Out of 16;8 ISSR primers, which included six di-and two tri-nucleotide primers amplified clear and polymorphic bands in 1-10 hybrids with their parents. The number of amplicons per primer ranged from 4 (CA1, CA2) to 16 (CA9) with an average 9.125 amplicons per primers. The average numbers of polymorphic bands per primer were 2.12. F1 purity was verified by the presence of parental specific band in each hybrid. The number of polymorphic bands ranged (2 to 4) but CA1, CA2, CA3 showed no polymorphic and parent specific bands. ISSR markers are beneficial and efficient for assessing the genetic purity of F1 hybrids and provide better opportunity for selection of superior genotypes for breeding purposes. This is the first report on the use of ISSR markers which provides the basis for authentication and identification of each of the progenitors of F1 hybrids of Capsicum annuum Land could be cited as ready reference for hybrid development and authentication in chillies.
To Cite this article:
Noor, T., A. Shahzad, N.N. Nawab, G.M. Ali and A. Ramzan. 2020. Authentication of indigenously developed chilli (Capsicum annuum L.) hybrids through ISSR markers. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(24)
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