PJB-2018-716
Molecular characterization of novel isolates of Rhizoctonia solani, Trichoderma atroviride and Fusarium spp. isolated from different plants and cutting woods in Iraq
Aqeel N. Al-Abedy, R.G. Al-Janabi, Z.A. Al-Tmeme, Alaa T. Salim and Muhammad Ashfaq
Abstract
Three fungal (Fusarium, Rhizoctonia and Trichoderma) species isolated from diseased tomato, maize and wood decayed plants from Iraq were morphologically identified. ITS1 and ITS4 based genetic regions were used for molecular identification and phylogenetic relationship revealed that that Rhizoctonia solani isolates had 99% similarities to Indian, USA and Mexicans isolates. Fusarium solani showed 99% nucleotide identity to Indian, Chinese, Mexicans and Brazilian isolates. Fusarium proliferatum showed >99% nucleotide identity to Asian isolates whereas Trichoderma atroviride showed 99% similarity to Chinese isolates. These identified fungal isolates of F. solani, F. proliferatum and T. atroviride were not previously reported from Iraq and therefore sequences of these fungi were deposited in the GenBank database (NCBI) under the accession numbers KY283953(R. solani), KX000895 (F. solani), KX350051 (F. proliferatum) and KU985151 (T. atroviride) for the first time.
To Cite this article:
Al-Abedy, A.N., R.G. Al-Janabi, Z.A. Al-Tmeme, A.T. Salim and M. Ashfaq. 2020. Molecular characterization of novel isolates of Rhizoctonia solani, Trichoderma atroviride and Fusarium spp. isolated from different plants and cutting woods in Iraq. Pak. J. Bot., 52(3): DOI: http://dx.doi.org/10.30848/PJB2020-3(9)
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