PJB-2018-762
Differential oxidative and biochemical responses of tomato and maize leaves to Spodoptera exigua herbivory
Manal El-Zohri, Sameera O. Bafeel and Wafaa Al-Zahrani
Abstract
Plants have been interacting with insects leading to complex defense approaches. Plant redox status could be regarded as an indicator for its resistance, which is accomplished by the elimination of generated ROS. In his study, we investigated the relationship between oxidative responses in tomato and maize after Spodoptera exigua attack and the plant tolerance. As an indication for plant resistance, fresh and dry biomass of tomato and maize shoot was determined. To investigate the oxidative responses, the concentration of hydrogen peroxide (H2O2), phenols and ascorbic acid (ASA) in addition to the activities of ascorbate peroxidase (APX), superoxide dismutase (SOD) and catalase (CAT) were assayed. All data were collected after 2 hours, 2 days and 1 week following infestation. The results indicated that, shoot fresh and dry weight of tomato significantly decreased lower than uninfected controls after 2 d and 1 week of infestation. However, maize shoot fresh and dry biomass decreased than the control only after 1 week of infestation. The infestation increases H2O2 and ASA concentration in maize leaves at 2 hours and 2 days after infestation while no change recorded in tomato leaves compared to their corresponding controls. Free phenols content increased in infested tomato leaves more than control. The infestation enhances SOD, CAT and APX activities in tomato and maize leaves. H2O2 content in the leaves of studied plants correlated with their differential tolerance responses. Therefore, it could be used as a diffusible signal to activate defensive genes in maize leaves, as recorded by increasing CAT and APX activities
To Cite this article:
El-Zohri, M., S.O. Bafeel and W. Al-Zahrani. 2020. Differential oxidative and biochemical responses of tomato and maize leaves to Spodoptera exigua herbivory. Pak. J. Bot., 52(4): DOI: http://dx.doi.org/10.30848/PJB2020-4(23)
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