PJB-2004-92
MAPPING QUANTITATIVE TRAIT LOCI (QTLS) FOR SALT TOLERANCE IN RICE (ORYZA SATIVA) USING RFLPS
M. SHAHID MASOOD, YANAGIHARA SEIJI*, ZABTA K. SHINWARI AND RASHID ANWAR
Abstract
Recombinant inbred (RI) lines derived from a cross between Tesanai 2 (moderately salt tolerant) and CB (salt sensitive) through single seed descent procedure were used to identify RFLP markers linked to QTLs involved in salinity tolerance. The RI population (F8) was evaluated for six different parameters using Yoshida's modified nutrient solution at an EC level of 12 dSm-1. The genotyping of 96 RI lines utilized 74 RFLP markers that revealed polymorphism. A linkage map was constructed from 12 linkage groups based on RI segregation data. The map covered 1349.5 cM of the rice genome with an average distance of 18.24cM between marker loci. Based on regression ANOVA (P = 0.05), one marker locus was found significantly associated with seedling survival days
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