PJB-2018-904
Cloning and characterization of Farnesyl pyrophosphate synthase gene from Anoectochilus
Lin Yang, Jun Cheng Zhang, Wan Chen Li, Jing Tao Qu, Hao Qiang Yu, Fu Xing Jiang and Feng Ling Fu
Abstract
Anoectochilus roxburghii and Anoectochilus formosanus are the two important species of genus Anoectochilus used as herbal drug and health food in traditional Chinese medicine for their abundant polysaccharides, glycoside derivative kinsenoside, and terpenoids. Farnesyl pyrophosphate synthase (FPS) catalyzes the key step of isoprenoid biosynthesis. Based on transcriptomic analysis, the open reading frame sequences of the FPS genes were obtained from A. roxburghii and A. formosanus. After bioinformatics analysis for high homology with their orthologs in related species and conserved domains of the FPS proteins, they were identified as the FPS genes of A. roxburghii and A. formosanus, respectively, and registered at GenBank. The FPS-eGFP fusion protein was specifically distributed in the cytoplasm and nucleus by transient expression in onion. Meanwhile, the expression of the FPS genes was detectable from leaf, root, and stem of these two species by real-time quantitative PCR, although their relative expression levels were significantly higher in root and stem. In response to high salt treatment, the expression of the FPS gene was downregulated in A. roxburghii and kept constant in A. formosanus. Under the illumination of red light, the downregulated range of the expression of the FPS gene in A. roxburghiiwas narower than that in A. formosanus. These results suggest that A. formosanus has higher tolerance to salt stress, and A. roxburghii is more suitable for cultivation under shade environment under forest.
To Cite this article:
Yang, L., J.C. Zhang, W.C. Li, J.T. Qu, H.Q. Yu, F.X. Jiang and F.L. Fu. 2020. Cloning and characterization of Farnesyl pyrophosphate synthase gene from Anoectochilus. Pak. J. Bot., 52(3): DOI: http://dx.doi.org/10.30848/PJB2020-3(14)
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