PJB-2019-61
Toxicological effect of CuO nanoparticles to Brassica nigra L. seedlings: a comparative in vivo and in vitro response
Hira Zafar, Ihsan-Ul-Haq, Syeda Nasreen and Muhammad Zia
Abstract
Metallic nanoparticles including CuO are considered toxic when released in the environment. However, the toxicity is concentration dependent. In present investigation, we report toxicological consequences of CuO nanoparticles under In vivo and In vitro conditions to Brassica nigra L (a model legume plant). To investigate LD50 (lethal dose), the seeds were germinated in presence of CuO NPs (1-1000 mg/L for In vitro and 10-200 mg/kg soil for In vivo condition). In both, In vitro and In vivo cases no or positive effect was observed on plant length, fresh and dry weight up to threshold concentration. Above that negative effect was observed on all physiological parameters. Under In vitro conditions Total phenolics and flavonoids (TPC and TFC), total antioxidative potential (TAC), total reducing power (TRP) and free radical scavenging activity fluctuated in Brassica plantlets by change in NPs concentration. While under In vivo application in Brassica plantlets TPC, TAC and TFC increased up to some concentration and then decreased while increasing the NPs concentration. Minor significant change was observed in TRP and DPPH based free radical scavenging activity continuously increased by increasing NPs concentration. HPLC analysis was performed for determination of rutin, gallic acid and caeffic acid in plants. All the three antioxidative molecules were found at varying concentration when plants were grown in presence of CuO NPs. The results conclude that under In vivo and In vitro condition NPs have different toxicity to plants which results in differential plant response to scavenge the free radicals and oxidative stress
To Cite this article:
Zafar, H. I.U. Haq, S. Nasreen and M. Zia. 2019. Toxicological effect of CuO nanoparticles to Brassica nigra L. seedlings: a comparative in vivo and in vitro response. Pak. J. Bot., 51(2): DOI: http://dx.doi.org/10.30848/PJB2019-2(15)
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