PJB-2018-967
Morpho-biochemical response of Vigna radiata to salinity generated hydrogen peroxide stress
Jabeen Farheen and Simeen Mansoor
Abstract
Salt stress is the most obvious limiting threat to the production of Vigna radiata due to the shortage of irrigated lands throughout the world. Thus, current investigation was conducted to appraise the growth performance and seedling survival of 96 hours old NM-92, NM-98, NM-51, NM13-1 and NM19-19 seedlings against salt stress. The whole study was planned in complete randomized design (CRD) in Petri dishes (n=4). Imbibed seeds (24 h) from all genotypes have received 72 hours of 0, 50, 150, 250 and 350 mM NaCl stress. All the five levels of salt gradually enhanced hydrogen peroxide (H2O2), and malondialdehyde (MDA), decreased seedlings length (SL), fresh biomass (BIO), and relative water content (RWC), superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT). Among genotypes, the maximum increase in growth and antioxidant enzymes were found in NM-92 seedlings at each concentration of NaCl. Additionally, NM-92 showed lowest H2O2 (10.1 μM gˉ1 FW), and MDA (11.2 μM gˉ1 FW) accumulation in its seedling tissues under 350 mM NaCl level. Compared with NM19-19 was shown least improvement in SL, BIO, RWC, SOD, POX, CAT, and greatest in H2O2, and MDA at five stress levels of NaCl. Hence, NM-92 could be designated as the salt tolerant genotype of Vigna radiata, and expected to give optimum yield over saline irrigated lands.
To Cite this article:
Farheen, J. and S. Mansoor. 2020. Morpho-biochemical response of Vigna radiata to salinity generated hydrogen peroxide stress. Pak. J. Bot., 52(4): DOI: http://dx.doi.org/10.30848/PJB2020-4(32)
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