PJB-2018-977
Smoke induced physiological, biochemical and molecular changes in germinating rice seeds
Muhammad Jamil, Muhammad Jahangir and Shafiq Ur Rehman
Abstract
Smoke produced by fire is an important environmental stimulus that plays a major role in improving the germination of plant species, including crops. However, smoke induced biochemical and molecular mechanisms involved in seed germination during imbibition remains unknown. Here, we explored the physiological, biochemical and molecular changes in the rice seed imbibed for 48 h in smoke-water (1:500 and 1:1000 dilutions (v/v)) , Gibberellic acid (GA3)(50 and 100µM) and Abscisic acid (ABA) (5 and 10µM). Increased the smoke concentration showed a significant increase in the germination percentage. It was also observed that smoke (1:1000) and GA3 (100µM) treated seeds had more water uptake as compared to other treatments. Interestingly smoke (1:1000) significantly induced carbohydrate, protein and lipid level of the imbibed seeds. However, macro and micro elements concentrations were decreased in seeds treated with smoke, GA3 and ABA as compared with seeds treated with water. Furthermore, the expression of GA3 and ABA responsive cis-elements genes was up-regulated by low or high dilution of smoke during seed imbibition, while the transcript abundance of some genes were up-regulated by GA3 at seedling stage.
To Cite this article:
Jamil, M., M. Jahangir and S.U. Rehman. 2020. Smoke induced physiological, biochemical and molecular changes in germinating rice seeds. Pak. J. Bot., 52(3): DOI: http://dx.doi.org/10.30848/PJB2020-3(37)
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