PJB-2019-88
Optimization of pectinase production from Geotrichum candidum AA15 using response surface methodology
Asia Ahmed, Muhammad Naseem Khan, Aqeel Ahmad, Shakeel Ahmed Khan and Muhammad Sohail
Abstract
Pectinolytic enzymes are used in industries involving in fruits and vegetables processing, wine making, retting and degumming of fiber crops, extraction of olive oil, treatment of pectin containing waste water and in the fermentation of coffee, tea and cocoa. Pectinase production from bacteria and fungi has been reported widely, however, very few reports are available for pectinolytic yeasts. This research was carried out to study the parameters affecting pectinase production from an indigenously isolated yeast strain, Geotrichum candidum AA15, using statistical tools. Plackett-Burman design revealed initial pH of the medium, inoculum size and substrate concentration as significant factors. Subsequently, optimum conditions for pectinase production were determined using Box-Behnken design. The data showed that the strain produced 0.250 IU/ml pectinase under optimized conditions using inoculum size, 3%; substrate concentration, 0.75%; and a starting pH of 5.3. Time course studies of the enzyme production revealed that the pectinase production was associated with the growth and optimum enzyme production was achieved after 48 h of cultivation. The presence of isozymes with approximate molecular weights of 60 and 66.2 kDa was indicated by SDS-PAGE and in-gel visualization of enzyme activity. These results provide a merit to study the strain further to explore its biotechnological potential.
To Cite this article:
Ahmed, A., M.N. Khan, A. Ahmad, S.A. Khan and M. Sohail. 2019. Optimization of pectinase production from Geotrichum candidum AA15 using response surface methodology. Pak. J. Bot., 51(2): DOI: http://dx.doi.org/10.30848/PJB2019-2(41)
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