PJB-2005-9
SOURCES OF GENETIC RESISTANCE IN MUNGBEAN AND BLACKGRAM AGAINST URDBEAN LEAF CRINKLE VIRUS (ULCV)
MUHAMMAD BASHIR, ZAHOOR AHMAD AND ABDUL GHAFOOR
Abstract
In order to identify sources of genetic resistance in mungbean and blackgram (mash), 32 accessions (16 each of mungbean and blackgram) were evaluated under greenhouse conditions by sap inoculation method. The inoculated plants of each accession were also tested by enzyme-linked immunosorbent assay (ELISA) using polyclonal antiserum to ULCV to separate the resistant plants from susceptible ones. From mungbean, only five genotypes viz., VC-3960 (A-88), VC-3960 (A-89), 98-CMH-016, NM-2 and BRM-195 were found highly resistant to ULCV. These genotypes neither expressed disease symptoms nor virus was detected by ELISA. However, in case of 98MG-003 genotype
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