PJB-2018-1008
Identification and heavy metals-induced expression analysis of the oligopeptide transporters (OPT) gene family in tomato
Muhammad Waseem and Iffat Shaheen
Abstract
The oligopeptide transporters are membrane integral proteins playing pivotal role in translocating secondary amino acids, small peptides, organic nitrogen mobilization and contribute in variety of biological activities. In this study, we have performed comprehensive bioinformatic identification of tomato OPT gene family and a total of 16 OPT genes were identified in tomato. These proteins were classified into two subfamilies including PT sub-family and YSL sub-family with eight genes in each based on phylogenetic analysis. Moreover, OPT exhibited a unique gene configuration that was consistent with previously reported studies and validate its phylogenetic classification. The putative OPT gene family members exhibited diverse expression pattern and few genes express in specific tissues. For example, SlYSL1, SlOPT3, and SlOPT5 expressed in flowers and SlOPT1, SlOPT2, and SlYSL2 in roots. However, some genes showed high expression in fruits at various developmental stages. These include SlOPT6, SlOPT8, SlYSL3, SlYSL4, and SlYSL5. It was observed that expression of some SlOPTs were induced under heavy metals such as calcium, cadmium, iron, magnesium, potassium, and lead in both root and shoot. This suggested a potential involvement of these genes in tomato adaptation under stress. In summary, our work is the first comprehensive analysis of oligopeptide transporters in tomato and an important resource for prioritizing genes especially in metal ions mobilizing and nutrient deficiency stress adaptation
To Cite this article:
Waseem, M. and I. Shaheen. 2020. Identification and heavy metals-induced expression analysis of the oligopeptide transporters (OPT) gene family in tomato. Pak. J. Bot., 52(5): DOI: http://dx.doi.org/10.30848/PJB2020-5(40)
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