PJB-2018-1032
Evaluation of fungicidal potential of Ocimum sanctum L. and Nicotiana tabacum L. against Aspergillus flavus and Aspergillus niger
Zara Naeem, Alya Maimoona, Khajista Jabeen, Bareera Khan and Sumera Iqbal
Abstract
The current study aimed to assess the antifungal potential of two medicinal plants (Nicotiana tabacum L. and Ocimum sanctum L. belonging to the Solanaceae and Lamiaceae families respectively against Aspergillus niger van Tieghem and Aspergillus flavus Link. Both plants were selected on the basis of their ethnobotanical uses. Dried test plants were dipped in methanol as the extraction solvent, and after getting filtrated by means of maceration procedure, their crude extracts were prepared. Methanolic crude extracts were then subjected to bioassay-guided fractionation to make various solvent fractions viz. n-hexane, dichloromethane, ethyl acetate and n-butanol. Phytochemical analysis was also carried out afterwards to confirm the absence or presence of secondary metabolites in test plants. Two concentrations i.e 0.1% and 0.3% of each fraction of O. sanctum and N. tabacum were prepared to conduct the antifungal assay. Results of antifungal test revealed that n-butanol fraction of N. tabacum had more potential in controlling the growth of A. flavus at 0.1 % in comparison to A. niger. On the other hand, O. sanctum proved to be efficient in the retardation of A. niger with a fraction of n-hexane (100%) at 0.3% concentration. Hence it is depicted by results that both of tested plants have vast potential to be used as antifungal agents
To Cite this article:
Naeem, Z., A. Maimoona, K. Jabeen, B. Khan and S. Iqbal. 2020. Evaluation of fungicidal potential of Ocimum sanctum L. and Nicotiana tabacum L. against Aspergillus flavus and Aspergillus niger. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(8)
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