PJB-2018-1078
Comparison among different auxins and cytokinins to induce date palm (Phoenix dactylifera L.) somatic embryogenesis from floral buds
Najamuddin Solangi, Adel Ahmed Abul-Soad, Ghulam Sarwar Markhand, Mushtaque Ahmed Jatoi, Tahira Jatt and Abdul Aziz Mirani
Abstract
The influence of different auxins and cytokinins combinations in inducing callus and somatic embryogenesis (SE) in two top commercial date palm cultivars of Pakistan Aseel and Dhakki were studied. In addition, the role of different concentrations of NaOCl (10%, 20%, 40% and 50%) in successful surface sterilization and as well as the influence of the spathes’ dimension (17, 28 and 32 cm in length) to induce callus were also investigated. The findings obtained showed varietal variation to induce somatic embryogenesis. The results obtained showed using 50% NaOCl solution produced significantly the lowest mortality and contamination percentages. The spikes’ explant obtained from immature inflorescence of 17 cm long and 6.2 cm in width gave significantly the highest callus formation in both cultivars. The MS basal medium supplemented with 2.0 mg L-1 2,4-D and 0.5 mg L-1 2iP formed white compact callus in the 87.3% of cv. Aseel and 84.3% of cv. Dhakki explants. On the other hand, the initiation medium provided with 2.0 mg L-1 NAA and 0.5 mg L-1 2iP significantly induced callus in cvs. Aseel (90.3%) and Dhakki (78%). Differentiation of the embryogenic callus occurred using 0.05 mg L-1 2,4-D and 2.0 mg L-1 2iP and the highest induction of non-repeated embryos (NRE) were achieved in most of cultures in cvs. Aseel (88.3%) and Dhakki (86%). But the direct shifting of embryogenic callus on PGRs free MS medium under full darkness after 9 months of initiation produced repeated embryos. These findings will expectedly help to define the appropriate size of spathe and plant growth regulators formula for somatic embryogenesis for commercial production of date palm.
To Cite this article:
Solangi, N., A.A. Abul-Soad, G.S. Markhand, M.A. Jatoi, T. Jatt and A.A. Mirani. 2020. Comparison among different auxins and cytokinins to induce date palm (Phoenix dactylifera L.) somatic embryogenesis from floral buds. Pak. J. Bot., 52(4): DOI: http://dx.doi.org/10.30848/PJB2020-4(30)
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