PJB-2018-1094
Phytochemical composition and potent biological activities of Ficus benjamina var. comosa leaves extract
Asma Ashraf, Sara Zafar, Muhammad Yasin Ashraf, Muhammad Umar Ijaz, Saima Muzammal, Farkhanda Asad, Farhat Jabeen and Muhammad Shahid
Abstract
Present work has been commenced to evaluate the possible antioxidant, antibacterial, hemolytic and antiviral properties of ethanolic extract of leaves of Ficus benjamina var. comosa. Total phenolic and flavonoid have also been estimated. DPPH free radicals scavenging method was used to assess antioxidant activity while anti-biofilm activity of the ethanolic leaves extracts was done by microtitre-plate assay against Pseudomonas aerugonisa, Escherichia coli and Bacillus cerus. For hemolytic activity the ethanolic extract toxicity was observed against human erythrocytes In vitro. In addition, SPF embryonated chicken eggs were used to check the antiviral activity against Newcastle disease virus. The values of total phenolic and flavonoid components were 4.50 ± 1.1 mg (GAE gm) and 11.20 ± 8.9 (QE mg) respectively. The results indicate that leaves have good antioxidant activity of 68.27 % ±1.08 with respect to ascorbic acid equivalence and biofilm inhibition against all the tested bacterial strains. Hemolytic activity is 2.14 % ±0.41 however at low concentration there was not any significant change observed on erythrocyte membrane. In antiviral In ovo activity 60 µg/mL showed complete inhibition of NDV growth while 120 µg/mL dose is lethal for embryo
To Cite this article:
Ashraf, A., S. Zafar, M.Y. Ashraf, M.U. Ijaz, S. Muzammal, F. Asad, F. Jabeen and M. Shahid. 2020. Phytochemical composition and potent biological activities of Ficus benjamina var. comosa leaves extract. Pak. J. Bot., 52(2): DOI: http://dx.doi.org/10.30848/PJB2020-2(3)
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