PJB-2018-1110
Molecular diversity of halophilic fungi isolated from mangroves ecosystem of Miani Hor, Balochistan, Pakistan
Shabir Ahmad Khan, Ali Akbar, Ponlada Permpornsakul, Benjawan Yanwisetpakdee, Xiaoming Chen, Muhammad Anwar and Imran Ali
Abstract
To find the halophilic fungal biodiversity hundred soil samples were collected from mangrove ecosystem of Miani-Hor Balochistan. Miani-Hor lagoon is about 50 km long with a single inlet from sea. Out of hundred soil samples 14 different halophilic and halotolerant microorganisms were isolated. Growth characteristics along with microscopy delineated diversity of the halophilic fungal isolates. To determine the molecular level identification and classification, DNA extraction and consequently sequencing was done. Molecular biodiversity was determined by internal transcribed spacer region of fungus. Molecular identification along with phylogenetic tree determination was achieved by using ITS regions of the species belonging to the genus Aspergillus (4 isolates), Penicillium (4 isolates), Alternaria (3 isolates), Fusarium (1 isolate) and Pleosporaceae (2 isolates). Diversified halophilic and halotolerant fungi from mangroves ecosystem in Pakistan will open doors for the application of indigenous species as a biorefineries.
To Cite this article:
Khan, S.A., A. Akbar, P. Permpornsakul, B. Yanwisetpakdee, X. Chen, M. Anwar and I. Ali. 2020. Molecular diversity of halophilic fungi isolated from mangroves ecosystem of Miani Hor, Balochistan, Pakistan. Pak. J. Bot., 52(5): DOI: http://dx.doi.org/10.30848/PJB2020-5(34)
Download
