PJB-2005-34
RAPD CHARACTERIZATION OF SOMACLONAL VARIATION IN INDICA BASMATI RICE
SULTANA RASHEED*, TAHIRA FATIMA, TAYYAB HUSNAIN, KHURRAM BASHIR AND SHIEKH RIAZUDDIN
Abstract
Seeds of three cultivars of indica basmati rice viz., B-370, B-2000 and Super basmati were sterilized and incubated for 2, 4, 6 and 8 weeks on callus induction medium. Fifteen interested regenerates were selected on the basis of variation in important morphological characteristics. DNAs from leaves of regenerated plants along with parental controls were isolated and analyzed through PCR using 8 Random Amplified Polymorphic DNA (RAPD) Primers. Four primers viz., S-13, S-19, R-17 and OPX-11 produced detectable range of amplification products and 7 interesting clones with different banding patterns were obtained. Index of genetic variation was calculated and dendrograms were constructed using UPGMA method. Genetic variation up to 45.2, 32.3, 32.4 & 35.3% was recorded after 8 weeks of incubation with primers S-13, S-19, R-17 and OPX-11 respectively. Our study indicates that tissue culture generates a wide range of variation, which is related with incubation time and is cultivar specific. Moreover, RAPDs can successfully be used to explore such polymorphism within and among different cultivars of basmati rice.
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