PJB-2005-35
USE OF SDS-PAGE MARKERS FOR DETERMINING QUANTITATIVE TRAITS LOCI IN BLACKGRAM [VIGNA MUNGO (L.) HEPPER] GERMPLASM
ABDUL GHAFOOR*, ZAHOOR AHMAD AND MUHAMMAD AFZAL
Abstract
One hundred and five genotypes of blackgram from diverse origin were evaluated for agronomic traits for 2 years and seed proteins were analyzed using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) through vertical slab type unit. Screening analysis for markers to quantitative traits revealed its significance in determining quantitative trait loci (QTL) in blackgram through SDS-PAGE markers. The factors affecting quantitative traits may occur as individual genes or gene clusters scattered throughout the genome, therefore, quantitative traits were expected differently at several loci. Variation in seven quantitative traits out of ten was significantly associated with 9 protein sub-units, however, the actual number of QTLs might be fewer because several of these traits were correlated. Variation at protein peptides in the vicinity of QTL in blackgram may be an indication of genetic variation potentially available to breeding programmes. Expansion of genetic base for blackgram breeding might be accomplished by systematic use of germplasm that differ from common banding pattern and known to be associated with variation in quantitative traits.
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