PJB-2019-377
Morphological and molecular charactererization of macro fungi from district Astore, Gilgit-Baltistan, Pakistan
Abdul Razaq, Sabela Shaheen, Maisoor Ahmad Nafees, Abdul Qayoom Rajput and Saleem Shahzad
Abstract
Present study is an attempt to estimate the genetic diversity of important macro fungi from district Astore using DNA based markers. The Province Gilgit-Baltistab represents a floristically rich area characterized by the moist and dry temperate forest region with rich macro fungal diversity. Duringthe field visits from March to August selected different localities of Astore were visited and collection was doneand subsequently identified macro fungal species on the basis of morphological and molecular characteristics viz., Bjerkandra adusta, Fomes fomentarius, Rigidoporus ulmarius and Tremetes versicolor were reported first time from Pakistan. Another ten species viz., Lepiota sistrata, Krombholziella scabra, Suillus leutus, Inocybe agardhii, Cyathus olla, Phallus impedicus, Schizophylum commune, Coprinus domesticus, Morchella esculenta and M. conica were reported first time from the study area. The fourteen fungal species were attended by eleven Randomly Amplified Polymorphic (RAPD) DNA primers. Bivariate data was used to estimate genetic diversity in different fourteen fungal accessions using unpaired group of arithmetic mean (UPGMA) procedure. High level genetic diversity ranging from 40% to 80% was observed in the fungal species. Samples of accessions were also grouped in to 7 clusters using dendrogram analysis
To Cite this article:
Razaq, A., S. Shaheen, M.A. Nafees, A.Q. Rajput and S. Shahzad. 2020. Morphological and molecular charactererization of macro fungi from district Astore, Gilgit-Baltistan, Pakistan. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(32)
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