PJB-2019-411
Establishment of real-time PCR for quantitatively monitoring of Pseudoperonospora cubensis in cucumber (Cucumis sativus)
Dong Liu, Chunqing Pan, Lei Tao, Di Zhang, Chunlong Wang, Ziwei He and Yanju Zhang
Abstract
Cucumber downy mildew caused by Pseudoperonospora cubensis is an important airborne disease in China and greatly threatens cucumber production. Early accurate quantitative detection is the foundation of forecasting and control. Here, we designed a pair of specific primers according to the rDNA-ITS sequence of P. cubensis in GenBank. These primers could amplify the DNA of P. cubensis isolates by conventional PCR but not the DNA of other pathogens.The sensitivity of real-time PCR was higher than that of conventional PCR.The real-time PCR detection results of infected cucumber samples were significantly positively correlated with disease indexes and inocula. In conclusion, real-time PCR assays for P. cubensis are fast, highly specific, sensitive, and reproducible. We therefore successfully established a SYBR Green I real-time PCR method for P. cubensis. This method can be used to detect P. cubensis in cucumber and help guide the prediction and control of cucumber downy mildew.
To Cite this article:
Liu, D., C. Pan, L. Tao, D. Zhang, C. Wang, Z. He and Y. Zhang. 2021. Establishment of real-time PCR for quantitatively monitoring of Pseudoperonospora cubensis in cucumber (Cucumis sativus). Pak. J. Bot., 53(3): DOI: http://dx.doi.org/10.30848/PJB2021-3(16)
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