PJB-2019-434
Morphological and biochemical characterization of rhizobacteria associated with peach plant and evaluation of these rhizobacteria against its fungal pathogens
M. Inam-Ul-Haq, Syed Afraz Ali, Gulshan Irshad, M Azam Khan and Sohaib Ismail
Abstract
Rhizospheric bacteria directly or indirectly affect plant growth. Management of plant diseases through biocontrol agents is one of the best approach that mayreduce the use of synthetic chemical based formulations. In this study, antagonistic activities of Bacillus subtiliswas evaluated In vitro against: Monilinia fructicola, Taphrina deformans and Alternaria alternate which cause Brown rot, Peach leaf curl and Alternaria rot. In dual culture technique, microbial antagonists inhibited the spore germination and mycelial growth of Moniliniafructicola and Alternaria alternata. Pathogenicity test for Taphrina deformance was also performed on leavesof Peach seedlings while pathogenicity of Monilinia fructicola and Alternaria alternata were done on Peach fruit being fruit rotting fungi. Out of 16 isolates,3 isolates B.subtilis viz., Rh3, Rh7 and Rh12 showed strongest activity against Monilinia fructicola, having 12mm, 12mm and9mm zone of inhibition for Taphrina deformans; 9mm, 9mm and 7mm and for Alternaria alternata and 8mm, 7.5 and 6mm zone of inhibition against. B. subtllis showed better results against Monilinia frecticola as compared to other pathogens Taphrinadeformans and Alternaria alternata. Many researchers are working on the biological control of fungal plant pathogens using antagonistic rhizo bacterial strains belonging to the genus Bacillus. The results of this study identify B. subtilis as encouraging biological control agents for further testing against Brown rot, Alternaria rot in fruit and peach leaf curl disease in peach leaves
To Cite this article:
Haq, M.I, S.A. Ali, G. Irshad, M.A. Khan and S. Ismail. 2020. Morphological and biochemical characterization of rhizobacteria associated with peach plant and evaluation of these rhizobacteria against its fungal pathogens. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(2)
Download
