PJB-2019-522
Assessment of phylogenetics relationship among the selected species of family Leguminosae based on chloroplast rps14 gene
Banzeer Ahsan Abbasi, Javed Iqbal and Tariq Mahmood
Abstract
The Leguminosae is a cosmopolitan family comprised of approximately 770 genera and 20,000 species mostly found in temperate and tropical regions of the world. This family is well known for its commercial, nutritional and medicinal importance. Twenty-six species of Leguminosae were collected from different geographical regions of Pakistan to resolve their controversial phylogenetic position using Ribosomal Protein Subunit (rps14) gene. Furthermore, in each species, the rps14 gene was successfully amplified, purified, sequenced and the dendrogram was constructed using molecular evolutionary genetic analysis (MEGA7) tool which divided the species into two main clades showing a narrow genetic diversity of 0.5 with well supported bootstrap values. Moreover, the range of pairwise distance was found to be 0.02 to 0.40 with a mean value of 0.15. The phylogenetic analysis is in agreement with the work done by earlier phylogenetists with the addition of new species, Crotalaria medicaginea, Argyrolobium roseum, Vicia sativa sub spp. nigra, Vicia sepium, Medicago falcata and Mimosa himalayana from Pakistan. The stereochemical analyses were evaluated and 3D protein structures were authenticated by Iterative Threading Assembly Refinement (I-TASSER) and Ramachandran plots. 3D protein models were quite good and can be reliably used for the application of different bioinformatics tools. Future studies can focus on the examination of morphology, anatomy, phylogenetics with multiple marker system and evolution of these and other variable characters within this diverse family.
To Cite this article:
Abbasi, B.A., J. Iqbal and T. Mahmood. 2021. Assessment of phylogenetics relationship among the selected species of family Leguminosae based on chloroplast rps14 gene. Pak. J. Bot., 53(4): DOI: http://dx.doi.org/10.30848/PJB2021-4(22)
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