PJB-2019-559
Endophytic radicular and rhizospheric microbiota associated with the endemic Cerrado palm, Butia archeri
Cintia Faria Da Silva, Luciana Cristina Vitorino, Liliane Cira Pinheiro, Kátia Aparecida De Siqueira, Marcos Antônio Soares and Edson Luiz Souchie
Abstract
Endophytic and rhizospheric microorganisms form complex communities that play pivotal role in plant growth and development, and contribute to the resilience of the host plant. As Butia archeri, a palm species endemic to the Cerrado savanna, is resistant to acidic soils and high concentrations of iron and aluminum, we decided to test the hypothesis that the endophyte and rhizosphere communities associated with this plant comprise microbiota rich in functional traits. To test this hypothesis, we isolated the cultivable radicular (endophytic) and rhizospheric microbiota of this palm and evaluated the capacity of the isolates to solubilize calcium (CaHPO4) and iron (FePO4) phosphates, synthesize indole acetic acid (IAA), and suppress the development of Aspergillus niger, the principal fungus known to deteriorate the seeds of this species. In total, 115 symbiotic bacterial and 17 fungal lineages were isolated, together with 40 seed-degrading fungi, primarily A. niger. The seed-deteriorating fungi presented a higher diversity index (Hꞌ = 1.82) than the other groups analyzed. Moreover, we confirmed the hypothesis that the microbiota associated with B. archeri has functional traits that contribute to plant growth. The BA81RB isolate of the bacterium Bacillus cereus solubilized 570.4 mg L−1 CaHPO4 and 750.2 mg L−1 FePO4. Furthermore, two isolates also solubilized remarkable amounts of CaHPO4, with the BA367EF and BA99RF lineages of Stagonosporopsis cucurbitacearum and Bionectria ochroleuca solubilizing 798.90 and 493.20 mg L−1 phosphorous, respectively. Additionally, we confirmed the presence of isolates that synthesized high concentrations of IAA, such as the BA147RB lineage of Enterobacter sp., which synthesized 97.0 μg mL−1, and others with potential for suppression of A. niger (BA68EB of Bacillus amyloliquefaciens and BA89RB of B. cereus). The present study provides novel insights into the symbiotic microorganisms associated with B. archeri, and reveals potentially important perspectives for the application of these isolates as promoters of plant growth, in particular for crops cultivated on the soils of the Cerrado biome.
To Cite this article:
Da Silva, C.F., L.C. Vitorino, L.C. Pinheiro, K.A. De Siqueira, M.A. Soares and E.L. Souchie. 2021. Endophytic radicular and rhizospheric microbiota associated with the endemic Cerrado palm, Butia archeri. Pak. J. Bot., 53(4): DOI: http://dx.doi.org/10.30848/PJB2021-4(23)
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