PJB-2019-592
Morpho-anatomical, histological, phytochemical and physicochemical characterization of Dicliptera bupleuroides Nees.
Shehla Akbar and Saiqa Ishtiaq
Abstract
Dicliptera bupleuroides Nees. a medicinal herb belongs to family Acanthaceae. Morpho-anatomical, phytochemical and physicochemical characterizations of Dicliptera bupleuroides Nees were determined. The anatomy of leaf, stem, and root depicted various structures including; fibres, vessels, tracheids, oil cells, starch granules, cortical cells, cork cells, phloem, collenchyma and parenchyma tissues etc. Anatomical sections of leaf, stem and root showed the arrangement of different cells, certain tissues that will serve as diagnostic characters to standardize this plant. In conjunction with these observations palisade ratio, stomatal index, vein termination, vein inlet number of leaf were also calculated. Histochemical features were studied by using Phlorogucinol, conc. HCl, Iodine solution, Ferric chloride and Sudan III solution. These reagents were used to locate the presence of Ca+2 oxalate crystals, lignin, starch, tannins and oil globules, respectively. In fluorescence analysis different colors were noted when powder of whole herb were exposed to ordinary and UV light. Phytochemical screening of methanolic extract of whole herb exhibited the occurrence of saponins, tannins, carbohydrates, flavonoids, glycosides, sterols, lipids and alkaloids. Physicochemical analysis i.e. extractive values and ash values were calculated to strengthen standardization process. These findings and estimations will help in characterization, verification and quality maintenance of Dicliptera bupleuroides Nees.
To Cite this article:
Akbar, S. and S. Ishtiaq. 2021. Morpho-anatomical, histological, phytochemical and physicochemical characterization of Dicliptera bupleuroides Nees. Pak. J. Bot., 53(3): DOI: http://dx.doi.org/10.30848/PJB2021-3(23)
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