PJB-2019-622
Identification and expression analysis of the auxin response factor (ARF) gene family in sorghum [Sorghum bicolor (L.) Moench]
Ling Zheng, Ji Zhou, Li Yi And Xiaojun Zhou
Abstract
Many ARF genes have been identified in plants, yet there remains a limitation of comprehensive analyses of this gene family in sorghum [Sorghum bicolor (L.) Moench]. In this study, we identified a total of 46 S. bicolor ARF (SbARF) genes by bioinformatics methods. Then, we analyzed this gene family in terms of conserved domain, phylogenetic relationship, chromosome location, gene structure, and expression pattern. The results showed that All SbARF genes could be divided into four subfamilies (classes I–IV) according to their relationship in Arabidopsis thaliana. Each of the SbARF genes consisted of one to 13 introns, with three highly conserved regions of the ARF, B3, and auxin/indole-3-acetic acid (Aux/IAA) domains, respectively. Class III genes were mainly expressed in 10 different tissues of sorghum, indicating important roles in growth of leaves, flowers and seeds. Expression levels of some SbARF genes were significantly upregulated or downregulated in roots or shoots of sorghum under stress induced by exogenous abscisic acid or polyethylene glycol, indicating critical roles in abiotic stress responses. This study provides insight into the role of the ARF gene family in the growth, development, and stress responses of sorghum.
To Cite this article:
Zheng, L., J. Zhou, L. Yi and X. Zhou. 2021. Identification and expression analysis of the auxin response factor (ARF) gene family in sorghum [Sorghum bicolor (L.) Moench]. Pak. J. Bot., 53(3): DOI: http://dx.doi.org/10.30848/PJB2021-3(37)
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