PJB-2019-693
In silico promoter characterization of PR genes and expression analysis in transgenic tomato plants expressing OsRGLP1
Aghogho Ohwofasa, Sumera Perveen, Tehmina Ahsen Khan, Bushra Ijaz and Tayyaba Yasmin
Abstract
Transgenic approaches have shown a huge potential in crop improvement against biotic and abiotic stresses. The regulated expression of stress induced genes provides insights about the underlying molecular mechanisms. In this study, we analyzed the expression of Pathogenesis related proteins in healthy transgenic tomato plants over expressing an important stress responsive gene (OsRGLP1). The OsRGLP1 was constitutively expressed in leaves and other parts of transgenic plants. We observed that OsRGLP1 brought about an increase in the expression of tomato PR2 and PR5 genes while no significant difference was observed in the expression of tomato PR1 gene in healthy transgenic tomato plants when compared with control. The promoter analysis of all three PR genes indicated that many conserved cis acting regulatory elements (CARES) were found in the promoters of all 3 PR genes analyzed. Furthermore, some CARES were common between all three PR genes and the promoter of OsRGLP1. This could possibly suggest that similar transcription factors are involved in the expression and regulation of all 4 genes. These results can improve our understanding of the regulation and expression of tomato PR1, PR2 and PR5 which can be geared towards improving the overall immunity of tomato plant to stress factors in relation with GLP genes.
To Cite this article:
Ohwofasa, A., S. Perveen, T.A. Khan, B. Ijaz and T. Yasmin. 2021. In silico promoter characterization of PR genes and expression analysis in transgenic tomato plants expressing OsRGLP1. Pak. J. Bot., 53(5): DOI: http://dx.doi.org/10.30848/PJB2021-5(36)
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