PJB-2019-729
Characterization of the genetic diversity of Epimedium brevicornum (Berberidaceae) via ISSR and CDDP markers
Yuanyuan Duan, Wuxian Zhou, Jingmao You, Xiaoliang Guo, Xiaorong Luo, Chao Lu and Jie Guo
Abstract
Epimedium brevicornum is a traditional and edible medicinal herb, used to tonify kidneys and strengthen tendons and bones. In this study, two types of molecular markers, namely, inter-simple sequence repeat (ISSR) and conserved DNA-derived polymorphism (CDDP) markers, were used to evaluate the genetic diversity of E. brevicornum. The results showed that both ISSR and CDDP markers were useful for estimating the genetic diversity. Forty-six polymorphic (86.79%) bands were generated by 7 random ISSR primers, while 82 polymorphic (95.35%) bands were generated by 11 CDDP primers. The genetic similarity coefficients of the ISSR markers ranged from 0.019 to 0.60, while those of the CDDP markers varied from 0.048 to 0.871. Moreover, the CDDP molecular marker technique was a preferable method for marker-assisted selection of stress-resistant varieties. Two superior resistant germplasms (BQ2 and MPC2) were screened, which would enable the selection and breeding of stress-resistant varieties of E. brevicornum
To Cite this article:
Duan, Y., W. Zhou, J. You, X. Guo, X. Luo, C. Lu and J. Guo. 2021. Characterization of the genetic diversity of Epimedium brevicornum (Berberidaceae) via ISSR and CDDP markers. Pak. J. Bot., 53(4): DOI: http://dx.doi.org/10.30848/PJB2021-4(44)
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