PJB-2020-23
Fingerprint characteristics and expression variability of miRNA based markers in wheat varieties with different susceptibility to drought
Katarina Razna, Angela Vargaova, Matus Kysel, Zdenka Galova and Jana Ziarovska
Abstract
Drought susceptible (Aladin, Dagmar) and drought tolerant (Seladon, Venturero) varieties of summer wheat were examined in experiment. Genotypes were tested under In vitro conditions on Murashige-Skoog culture medium with different concentrations (0, 5, 10, 15 and 20 %) of polyethylene glycol to induce dehydration stress conditions. Genomic response of seedlings was tested by stress-sensitive miRNA markers, tae-miR156, tae-miR408 and tae-miR827. In general, the fingerprints generate activity of tae-miR156 was higher in resistant genotypes in comparison to susceptible ones and lower in tae-miR408. The genome response to induced dehydration stress was genotype-specific, what has been statistically proven. The phosphate-induced tae-miR827 marker activity was balanced, since the optimal nutritional composition of the medium, create appropriate conditions to cope with the stress factor. The expression levels of analysed miRNA correspond to the differences in obtained fingerprints what confirmed the DNA based analysis of miRNAs markers as a very good screening tool for their analysis in plat genomes
To Cite this article:
Razna, K., A. Vargaova, M. Kysel, Z. Galova and J. Ziarovska. 2023. Fingerprint characteristics and expression variability of miRNA based markers in wheat varieties with different susceptibility to drought. Pak. J. Bot., 55(1): DOI: http://dx.doi.org/10.30848/PJB2023-1(35)
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