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Year 2001 , Volume  33, Issue 2
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S.No. Title Authors Pages Download
1
EFFECT OF SOME SALTS ON IN VITRO GROWTH OF FUSARIUM SOLANI
HINA FIRDOUS AND SALEEM SHAHZAD

EFFECT OF SOME SALTS ON IN VITRO GROWTH OF FUSARIUM SOLANI
ABSTRACT:
Effect of different salts viz., sea salt, NaCl , CaCl2, NaNO3, MgSO4, MgCl2 and KCl was studied on in vitro growth of Fusarium solani by food poison method. Growth of F. solani was enhanced as compared to control in potato sucrose agar (PSA) medium containing sea salt @ 20,000 ppm but suppressed where sea salt was used @ 60,000 ppm or more. No conidia were produced in 100,000 ppm of sea salt. Similarly, F. solani showed enhanced growth in NaCl at 10,000 and 20,000 ppm and declined with increasing conc. from 30,000 to 100,000 ppm. Growth of F. solani also increased significantly with increasing conc. of MgSO4 up to 40,000 ppm but declined thereafter. Use of NaNO3 @ 10,000 to 70,000 ppm showed significant increase in growth with massive cottony white mycelium as compared to control. Growth of F. solani declined gradually compared to control with increasing conc. of MgCl2 from 20,000 ppm to 100,000 ppm. Similarly, use of KCl @ 20,000 to 40,000 ppm showed gradual decline in growth of F. solani whereas @ 50,000 ppm or more, growth started after 7-days of inoculation. Amendment of PSA with CaCl2 showed gradual decline in growth of F. solani with increasing conc. of CaCl2. Chlamydospores were produced only where NaNO3 was used @ 10,000 to 40,000 ppm.

117-124 Download
2
PROTOPLAST ISOLATION IN SUGARCANE
IMTIAZ AHMED KHAN, ABDULLAH KHATRI , MUHAMMAD ASLAM JAVED, SHAMIM HUSSAIN SIDDIQUI, MAQBOOL AHMAD, NAZIR AHMED DAHAR, MUHAMMAD HUSSAIN KHANZADA AND RAZIULLAH KHAN

PROTOPLAST ISOLATION IN SUGARCANE
ABSTRACT:
A protocol was established for isolation of sugarcane protoplasts, cell wall regeneration and cell division. Protoplast were isolated from cell suspension initiated from fresh leaf calli and produced on agar medium by enzymatic treatment, 4% cellulase

125-132 Download
3
A MORPHOLOGIC AND TAXONOMIC STUDY OF HORDEUM MURINUM SENSU LATO (POACEAE: TRITICEAE) IN IRAN
JAMAL SAHEBI, AHMAD GHAHREMAN* AND MOHAMMAD REZA RAHIMINEJAD

A MORPHOLOGIC AND TAXONOMIC STUDY OF HORDEUM MURINUM SENSU LATO (POACEAE: TRITICEAE) IN IRAN
ABSTRACT:
Quantitative, qualitative and multi-state morphological characters on 350 herbarium specimens from 35 Iranian populations of Hordeum murinum s.l. were examined. Based on the results of this study, the followings were chosen as diagnostic characters: fresh leaf colour, inflorescence density, inflorescence colour at anthesis and maturity, pedicel presence in central spikelet and central floret, ratio of the length of the central spikelet (excluding awn) to that of the lateral ones, shape and colour of the lateral spikelets rachilla prolongation, central spikelet lodicules margin vestiture, ratio of the anther length of the central floret to those of the lateral ones, central and lateral florets anther colour, central floret anthers situation at anthesis, and lateral florets palea vestiture. Regarding morphological diversity, the idea that this group has three distinct species is confirmed. Moreover, Hordeum murinum L., is recorded for the first time from Iran from Bandar-e Abbas and Bandar-e Lengeh. Using morphological characters, two taxonomic keys are provided for the taxa in Hordeum murinum s.l.

133-141 Download
4
NOTES ON THE GENUS VERONICA (SCROPHULARIACEAE: TRIBE VERONICEAE) IN IRAN: SEED CHARACTERS AND A NEW RECORD
SHAHRYAR SAEIDI-MEHRVARZ, AHMAD GHAHREMAN* AND MOSTAFA ASSADI**

NOTES ON THE GENUS VERONICA (SCROPHULARIACEAE: TRIBE VERONICEAE) IN IRAN: SEED CHARACTERS AND A NEW RECORD
ABSTRACT:
The seed characters of 10 species of Veronica in Iran are described. Six distinct types based on seed features such as shape, surface ornamentation and cell shape are distinguished. A diagnostic key for determination of these taxa is provided. One of the examined species i.e., Veronica davisii, is recorded for first time from Iran. This species is compared with its closest relatives within sect. Veronicastntrum.

143-152 Download
5
A SHORT CONTRIBUTION TO GENUS ASTRAGALUS L. (FABACEAE) IN TURKEY
SHAHIN ZARRE AND DIETER PODLECH1

A SHORT CONTRIBUTION TO GENUS ASTRAGALUS L. (FABACEAE) IN TURKEY
ABSTRACT:
Through selecting new epitypes for incomplete type material of both A. cryptocarpos and A. rechingeri the systematic position of Astragalus cryptocarpos is clarified. It was confirmed that the latter must be reduced under synonymy of the former. Scaning-electron microscopy showed that A. cryptocarpos belongs to A. sect. Acanthophace.

153-155 Download
6
SOME INTERESTING ENDEMIC PLANTS COLLECTED FROM THE EAST AND THE NORTHEAST OF TURKEY AND THEIR THREATENED CATEGORIES
YAS├ŁN ALTAN

SOME INTERESTING ENDEMIC PLANTS COLLECTED FROM THE EAST AND THE NORTHEAST OF TURKEY AND THEIR THREATENED CATEGORIES
ABSTRACT:
This study was carried out during the field trips for Turkey's endemic plant seeds collection project where 48 taxa presented in this study were interesting in terms of being known from the type-gathering, their collection long years ago and as topotype, their distribution in very small area, providing data of unknown morphological characteristics in the Flora of Turkey. They are also presented with their threatened categories.

157-166 Download
7
KARYOLOGICAL STUDY ON SOME ENDEMIC IRIS L. (IRIDACEAE) SPECIES FROM TURKEY
MUSTAFA ÖZKAN, NEZAHAT KANDEMIR AND GÜLCAN SENEL

KARYOLOGICAL STUDY ON SOME ENDEMIC IRIS L. (IRIDACEAE) SPECIES FROM TURKEY
ABSTRACT:
Karyotype structure of two Iris L., species viz., Iris galatica Siehe and Iris kerneriana Ascherson & Sint ex Baker collected from Central Black Sea Region of Turkey have been analysed. Chromosome number determined is I. galatica 2n=24, I. kerneriana 2n=18.

167-171 Download
8
CHARACTERIZATION OF MUSTARD [BRASSICA JUNCEA (L.) CZERN. & COSS.] GERMPLASM BY SDS-PAGE OF TOTAL SEED PROTEINS
M. A. RABBANI, A.A. QURESHI, M. AFZAL, R. ANWAR AND S. KOMATSU*

CHARACTERIZATION OF MUSTARD [BRASSICA JUNCEA (L.) CZERN. & COSS.] GERMPLASM BY SDS-PAGE OF TOTAL SEED PROTEINS
ABSTRACT:
A comparative study of total seed storage protein was carried out by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to characterize oilseed mustard [Brassica juncea (L.) Czern. & Coss.] germplasm from Pakistan. Oilseed collections from Pakistan as well as oilseed cultivars from diverse origin were not differentiated from each other nor were vegetable cultivars found to be distinct from one another on the basis of their seed protein patterns. Eight types of protein were recognized based on the banding patterns of 52 accessions. Relative inter-type relationships of protein patterns were estimated using Jaccard's similarity index and a dendrogram showing the hierarchical clustering was constructed by unweighted pair-group method with arithmetic averages (UPGMA). The clustering of eight protein types generally agreed with our previous classification and the limited data already available on inter-accession relationships in oilseed mustard based on morphological traits and RAPD analysis. The differences between the profiles of accessions 'PAK-85835', 'PAK-85839' and 'PAK-85910' supported the idea that they were separate species from B. juncea. The results showed that the technique of SDS-PAGE applied to seed proteins was not feasible to distinguish the closely related oilseed collections and cultivars from each other as they were characterized by the same banding pattern and formed a common gene-pool. However, seed proteins were useful to discriminate B. juncea and B. campestris. It was also possible to distinguish the oilseed mustard from the vegetable form. Future studies should involve a greater number of local accessions of oilseed mustard from other parts of Pakistan, and those of vegetable and condiment forms to further elucidate the situation.

173-179 Download
9
STUDIES ON INHERITANCE OF SOME ISOZYMES IN LENTIL (LENS CULINARIS)
M. EHTISHAM-UL-HAQ, M. MUNIR, BASHIR A. MALIK AND NASIR M. MINHAS

STUDIES ON INHERITANCE OF SOME ISOZYMES IN LENTIL (LENS CULINARIS)
ABSTRACT:
Inheritance of three isozymes i.e., Leucine aminopeptidase-1, Phosphoglucoisomerase-1, and Phosphoglucomutase-2 in lentil was investigated. The plants were grown in greenhouse and the isozyme analysis was done by starch gel electrophoresis. X2 procedure was applied to test the goodness-of-fit for the observed and hypothesized ratios. All the three isozymes showed a monogenic inheritance pattern with co-dominant alleles for fast and slow allozymes. Heterozygous plants can be recognized at early stages and selfed plants may be discarded immediately. Their use in lentil improvement has been discussed.

181-186 Download
10
GENOTYPE ENVIRONMENT INTERACTION IN MUNGBEAN
MUHAMMAD ZUBAIR AND ABDUL GHAFOOR

GENOTYPE ENVIRONMENT INTERACTION IN MUNGBEAN
ABSTRACT:
Twelve genotypes of mungbean (No. 7-2, NCM 201, NCM 209, No. 1, NCM 205, AEM 6/20, NM 18, AEM 30/20, NM 93, NM 90, NM 121-25, M 20-24) were evaluated for stability of grain yield under seven diverse environments of Pakistan. The interaction between the genotypes and environments (G X E interaction) was used as an index to determine the yield stability of genotypes under all the environments. The G X E interaction was highly significant and both linear as well as non-linear components were equally important for determining the yield stability. The genotype "NM 90" gave the highest yield with above average regression value and non-significant standard deviation, showing consistently better performance in favorable environments. The genotype "NCM 209" also had high mean yield over the environments and did extremely well under less favorable conditions. The genotypes "No.1" and "NM 18" responded well under favorable conditions as compared to unfavorable conditions. The genotypes "NCM 201", "M 20-24" and "AEM 30/20" had below average yield, whereas their stability parameters revealed above average stability with specific adaptation to unfavorable environments.

187-190 Download
11
STABILITY ANALYSIS FOR POD YIELD IN GROUNDNUT (ARACHIS HYPOGAEA L.)
NAAZAR ALI, MALIK SHAH NAWAZ, M. YASIN MIRZA AND GHULAM RAZA HAZARA

STABILITY ANALYSIS FOR POD YIELD IN GROUNDNUT (ARACHIS HYPOGAEA L.)
ABSTRACT:
Ten promising genotypes of groundnut were evaluated for their adaptiveness and stability performance at eight different locations during 1997. The G x E interaction was highly significant. The regression coefficients (b) ranged from 0.76 to 1.17 and deviations from regression (S2d) ranged from 0.06 to 0.82. BM-28 and ICGV-86550 showed above average performance, unit slop and non significant deviation from regression and thus are suitable for wide range of environments. Four genotypes, Gori, ICG-7326, CINA and Chinese 68-4 possessed b < 1 and mean performance was also below average hence are suitable for poor environment. Out of all the tested genotypes, ICG-4993 had greater than unit regression coefficient (bi=1.14) and highest significant (S2d) (0.82) indicating its unsuitability for wider cultivation.

191-196 Download
12
EFFECTS OF DROUGHT STRESS ON ABSCISIC ACID, MANNITOL, K+ AND Ca2+ CONTENT IN TWO LINES OF WHEAT (TRITICUM AESTIVUM L.)
R. ASGHARI, H. EBRAHIMZADEH*AND A. R. KHABIRI**

EFFECTS OF DROUGHT STRESS ON ABSCISIC ACID, MANNITOL, K+ AND Ca2+ CONTENT IN TWO LINES OF WHEAT (TRITICUM AESTIVUM L.)
ABSTRACT:
Experiments were conducted to determine the level of ABA, K+ and Ca2+ mineral content and mannitol in the leaves of two lines of wheat (Triticum aestivum L.) under field condition in order to find any relationship between them and the tolerance of the plant against drought stress and to know if we can use one or more of them in determining tolerant or sensitive plant. The resistance of the plants was affected by the studied factors and all of them changed under drought conditions. Both K+ and Ca2+ have an important role in determining the stomatal situation, however, the ratio of K+/Ca2+ may regulate the position of stomata in different conditions. The existence of an inverse relation between this ratio and the rate of mannitol confirms the validity of this hypothesis. When the ratio is low, stomata are closed and when it is higher than the control, the stomata are open. Thus we could suggest this ratio to determine tolerant and sensitive lines, as well as the tolerant or sensitive stages of the plant development.

197-202 Download
13
IRREVERSIBLE INACTIVATION OF A TRANSGENE IN ARABIDOPSIS THALIANA
BUSHRA MIRZA

IRREVERSIBLE INACTIVATION OF A TRANSGENE IN ARABIDOPSIS THALIANA
ABSTRACT:
Plant transformation is now a routine practice in many laboratories around the world. However it has been observed that sometimes the introduced gene becomes inactive. This inactivation could be reversible or irreversible. In this study the genetic behavior of an inactive transgene was observed. For this purpose a wild type chlorophyll gene CH-42 was introduced into a pale (ch-42) mutant Arabidopsis by Agrobacterium mediated transformation. After transformation most of the plants showed green wild type phenotype. However, some transgenic lines did not show the phenotype of the CH-42 transgene and appeared pale in color. Four transgenic lines showing inactive CH-42 transgene were selected to study the segregation of the inactive transgene in the next generations. These transgenic lines with inactive insert were crossed to the wildtype and the CH-42 mutant plants. The F1 plants produced from these crosses were allowed to self fertilize and some of the seeds of these F1 were germinated for analysis of the F2 generation. The results of the reciprocal crosses of all four inactive lines were similar. The inactivation of transgene CH-42 was irreversible and the cellular gene at the CH-42 locus did not have any effect on the expression of the transgene.

203-209 Download
14
NUCELLAR REGENERATION AND POLYEMBRYONY OF CITRUS CULTIVARS
NAFEES ALTAF, E.K. MURWAT, I.A. BHATTI AND M. MOHSIN IQBAL

NUCELLAR REGENERATION AND POLYEMBRYONY OF CITRUS CULTIVARS
ABSTRACT:
Thirty four Citrus cultivars were studied for nucellar embryogenesis and for the extent of polyembryony per seed. Ovule weight had no correlation with the number of nucellar embryos. Also seed weight had no correlation with the number of embryos per seed. The maximum and minimum number of embryos per nucellus were in cvs. Kinnow (7.75) and Baramashi (2.23), respectively. Secondary somatic embryogenesis was also studied which was maximum in cv. Kinnow (8.40) and minimum in cv. Baramashi (2.90). Maximum and minimum polyembryony were in cultivars Minneola (7.50) and Orlando (2.75), respectively which indicated that nucellar embryogenesis was cultivar dependent. The soil survival after two months hardening process was maximum in cvs. Minneola (100 %), Shamber (100 %), Foster (100 %), Gada dehi (100 %) and minimum in cv. Baramashi (10.00%).

211-215 Download
15
FUNGI ASSOCIATED WITH ROOT AND STEM OF BETELVINE IN PAKISTAN
SALEEM SHAHZAD

FUNGI ASSOCIATED WITH ROOT AND STEM OF BETELVINE IN PAKISTAN
ABSTRACT:
A survey of betelvine fields in Karachi and Thatta districts of Sindh and Hub area of Balochistan was conducted during 1996 to 1999 where 38 funfal species belonging to 25 genera were isolated from root and stem of diseased betelvine plants. Of the fungi isolated

217-219 Download
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