PJB-2018-353
Protein isolation and identification of trichosanthes kirilowii seed from different places of production
Jin Xie, Xiaoqi Zhu, Rong Song, Siwen Peng and Yangning Huang
Abstract
In this paper, 12 samples of Trichosanthes kirilowii seed from different places of production were studied. The total proteins of the 12 samples were extracted by Tris-phenol method. Twelve samples of proteins were isolated by SDS-PAGE. SDS-PAGE results showed that the protein bands in sample No.8 are different from those of other samples. And those protein bands were identified by mass spectrometry. At least 10 differentially expressed proteins were identified, including rRNA N-glycosidase, Acetyl-CoA acyltransferase, Citrate synthase, Phosphoglycerate kinase, Isocitrate dehydrogenase, Fructose-bisphosphate aldolase, Ferritin, Urease, Hhydroxyacyl-ACP-dehydratase, Glutathione peroxidase. From the result of protein identification, it can be inferred that the seed of No. 8 sample was superior to other samples during germination
To Cite this article:
Xie, J., X. Zhu, R. Song, S. Peng and Y. Huang. 2020. Protein isolation and identification of trichosanthes kirilowii seed from different places of production. Pak. J. Bot., 52(1): DOI: http://dx.doi.org/10.30848/PJB2020-1(29)
Download
