Paper Details

PJB-2024-313

Genome-wide identification and expression analysis of early flowering (ELF) gene family in Nicotiana tabacum

Yudong Chen, Yanju Shuai, Wenyuan Wang, Lele Deng, Haitao Hang, Zhong Wang, Wanli Zeng, Kangkang Song and Qian Gao
Abstract

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Early Flowering (ELF) genes are crucial in plant photoperiod pathways and are negative regulation genes of plant flowering. At present, the identification of ELFs in Nicotiana tabacum (NtELFs) has not been systematically carried out. Using the Arabidopsis ELF family genes as a reference, 28 NtELF family members were identified, which were divided into four subfamilies. The ELF4 subfamily exhibited the highest membership counts of 13 members and owned a unique motif 1; with the exception of NtELF4-9. All members of the ELF4 subfamily had only one CDS. The results of chromosome localization of the ELF family members showed that there were three sets of tandem repeat events in the NtELFs. Promoters of NtELFs contained four cis-acting element types, and the light response elements were significant abundance. The NtELF family proteins within the same subfamily exposed comparable 3D structures and demonstrated analogous gene expression patterns. GO enrichment analysis of NtELFs showed that terms related to stimulus, photoperiodism, and reproduction were significantly enriched. This study systematically identifies NtELF family members for the first time, thereby paves the way for further investigations into their biological functions and provides a valuable background information for gene editing breeding

To Cite this article: Chen, Y., Y. Shuai, W. Wang, L. Deng, H. Hang, Z. Wang, W. Zeng, K. Song and Q. Gao. 2026. Genome-wide identification and expression analysis of early flowering (ELF) gene family in Nicotiana tabacum. Pak. J. Bot., 58(5): DOI: http://dx.doi.org/10.30848/PJB2026-5(5)  
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