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Year 2003 , Volume  35, Issue 3
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S.No. Title Authors Pages Download
1
POLLEN FLORA OF PAKISTAN - XXXV. BUTOMACEAE
ANJUM PERVEEN AND M. QAISER

POLLEN FLORA OF PAKISTAN - XXXV. BUTOMACEAE
ABSTRACT:
Pollen morphology of the family Butomaceae has been examined from Pakistan by light and scanning electron microscope. Pollen grains are generally heteropolar, boatshaped, mono-colpate with reticulate tectum.

257-259 Download
2
TAXONOMIC RELATIONSHIP OF THE GENUS DIGITARIA IN PAKISTAN
SYED SHAHINSHAH GILANI*, MIR AJAB KHAN**, ZABTA KHAN SHINWARI***, FARRUKH HUSSAIN AND ZUBAIDA YOUSAF**

TAXONOMIC RELATIONSHIP OF THE GENUS DIGITARIA IN PAKISTAN
ABSTRACT:
Numerical techniques were used to show the taxonomic relationship between the Digitaria species in Pakistan. A correlation phenogram showed a distinct dissimilarity between the species.

261-278 Download
3
A NEW SUBSPECIES OF DIGITARIA SANGUINALIS FROM PAKISTAN
SYED SHAHINSHAH GILANI, MIR AJAB KHAN* , ZABTA KHAN SHINWARI** AND ANWAR NASIM**

A NEW SUBSPECIES OF DIGITARIA SANGUINALIS FROM PAKISTAN
ABSTRACT:
A new subspecies with two varieties of Digitaria sanguinalis as D. sanguinalis ssp. pakistanica var. pakistanica and var. stewartii is identified. D. sanguinalis has spines on the whole nerves of the lower lemma while the new subspecies is found to have spines on the upper half margins of nerves of the lower lemma only. It is also a distinguishing character. The lower lemma of the spikelet of variety pakistanica is glabrous while that of variety stewartii is hairy.

279-282 Download
4
GYMNOSPERMS OF THE WESTERN HIMALAYA. 1. THE GENUS JUNIPERUS (CUPRESSACEAE)
G.H. DAR AND K.I. CHRISTENSEN*

GYMNOSPERMS OF THE WESTERN HIMALAYA. 1. THE GENUS JUNIPERUS (CUPRESSACEAE)
ABSTRACT:
A thorough study of an extensive collection of herbarium specimens and literature of Juniperus (Cupressaceae) from the Western Himalaya, during our work on gymnosperms of this region, has revealed that the taxonomy of West Himalayan Junipers has been confusing. A total of up to 6 taxa have been reported from this region by various earlier workers under a large number of specific and infraspecific names, most of which are synonyms. Seven taxa are recognised from the Western Himalaya in the present study: one belonging to Juniperus Sect. Juniperus, J. communis var. saxatilis, and the other six to J. Sect. Sabina. The latter section includes two acicular-leaved species, J. squamata and J. recurva, and four scale-leaved species: two multiseed, J. semiglobosa and J. polycarpos, and two monoseed, J. wallichiana and J. pseudosabina.

283-311 Download
5
FRUIT AND SEED MORPHOLOGY IN IRANIAN SPECIES OF FRITILLARIA SUBGENUS FRITILLARIA (LILIACEAE)
GHOLAMREZA BAKHSHI KHANIKI

FRUIT AND SEED MORPHOLOGY IN IRANIAN SPECIES OF FRITILLARIA SUBGENUS FRITILLARIA (LILIACEAE)
ABSTRACT:
Fruits and seeds of all Iranian taxa of Fritillaria subgenus Fritillaria were investigated by scanning electron and light microscopy. The fruit in Fritillaria is a three-valved loculicidal capsule, longitudinal dehiscence occurs down the centre of the outer walls of the three compartments or loculi. The capsule is wingless and the seeds are numerous in each capsule, flattened and ± membranously-margined. They are often broadly ovate in outline. The testa cells have irregular shape with undulate margins.

313-322 Download
6
MOLECULAR PHYLOGENY OF SEVEN DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS BY DNA FINGERPRINTING
EJAZ ASKARI, NASSER S. AL-KHALIFA, TAKEFUMI OHMURA, YOUSEF S. AL-HAFEDH, FAIQ A. KHAN, ABDULMANNAN AL-HINDI AND RYOJI OKAWARA

MOLECULAR PHYLOGENY OF SEVEN DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS BY DNA FINGERPRINTING
ABSTRACT:
Random Amplified Polymorphic DNA (RAPD) was used to detect polymorphism among the 7 different cultivars of date palm (Phoenix dactylifera L.) from the Kingdom of Saudi Arabia. Total genomic DNA extracted from young sprouting leaves (white to pale yellow) were amplified using random sequenced 10-mer OPERON primers. Out of 140 primers initially screened, 42 detected polymorphism among the cultivars. A total of 213 bands were generated with an average of 5.6 RAPD markers per primer. Out of a total 213 amplified fragments

323-330 Download
7
EFFECT OF CULTURE MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF PEACH ROOTSTOCK GF 677
TOUQEER AHMAD, HAFEEZ-UR-RAHMAN, CH. M. S. AHMED* AND M. H. LAGHARI

EFFECT OF CULTURE MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF PEACH ROOTSTOCK GF 677
ABSTRACT:
The effects of Murashige & Skoog (1962) and Anderson (1984) media with different BA (benzyladenine) concentrations (0.3, 0.6, 0.9 mg l-1) on the in vitro shoot proliferation of peach rootstock GF 677 was investigated. Shoot proliferation, elongation and growth on MS medium was the best whereas on AND medium the shoots were chlorotic, small and vitrified. BAP 0.6 mg l-1 produced higher number of shoots having > 2 cm length. Higher levels of BAP (0.9 mg l-1) induced callus formation and shoot apical necrosis. The best root system was developed on half strength MS media supplemented with 3 mg l-1 indolebutyric acid (IBA). Higher levels of IBA (4.0 mg l-1) induced callus and inhibited normal root development.

331-338 Download
8
TOWARDS A SEEDLESS CULTIVAR OF KINNOW MANDARIN III.VARIABILITY OF DEVELOPED AND UNDEVELOPED SEED NUMBER IN SEEDLESS/LOW SEEDED FRUITS
NAFEES ALTAF AND M. MOHSIN IQBAL

TOWARDS A SEEDLESS CULTIVAR OF KINNOW MANDARIN III.VARIABILITY OF DEVELOPED AND UNDEVELOPED SEED NUMBER IN SEEDLESS/LOW SEEDED FRUITS
ABSTRACT:
Seedless trait is desirable and was selected on the basis of fruit stylar ring which has 5 - 20% probability of carrying seedless trait and narrow new emerging leaves of sprouts and shoots of branches bearing seedless/low seeded fruits. Fruit with stylar ring has notched narrow leaves at the apex of sprouts.

339-342 Download
9
TOWARDS A SEEDLESS CULTIVAR OF KINNOW MANDARIN IV. EMBRYOGENESIS OF 1-12 SEEDED FRUITS
NAFEES ALTAF AND M. MOHSIN IQBAL

TOWARDS A SEEDLESS CULTIVAR OF KINNOW MANDARIN IV. EMBRYOGENESIS OF 1-12 SEEDED FRUITS
ABSTRACT:
Kinnow mandarin has nucellar polyembryony. Embryogenesis of fruits having 1-12 seeds was studied. There were 24 types of embryonic structures with most normal as 2-3 leaf with balanced germination. The normal looking embryos have best plant growth while meristemless embryos have no survival upon top - grafting on rootstock seedlings.

343-350 Download
10
ROOT, STEM AND LEAF ANATOMY OF ABUTILON THEOPHRASTII MEDIK. (MALVACEAE)
AYSEGÜL GÜVENÇ, AYSE MINE ÖZKAN, CEYDA SIBEL ERDURAK AND MAKSUT COSKUN

ROOT, STEM AND LEAF ANATOMY OF ABUTILON THEOPHRASTII MEDIK. (MALVACEAE)
ABSTRACT:
Abutilon theophrastii Medik., used in folk medicine in Turkey has been investigated anatomically. Examinations were done on transverse sections of leaf, stem and root.

351-359 Download
11
A VALIDATED LEAF AREA PREDICTION MODEL FOR SOME CHERRY CULTIVARS IN TURKEY
HUSNU DEMIRSOY* AND LEYLA DEMIRSOY

A VALIDATED LEAF AREA PREDICTION MODEL FOR SOME CHERRY CULTIVARS IN TURKEY
ABSTRACT:
A Leaf Area Prediction Model was developed for 12 cherry cultivars viz., (Hüsenba [1], Lambert [2], 0900 Ziraat [3], Van [4], Bing [5], Bella di Pistoia [6], Stella [7], Early Burlat [8], Karakirtik [9], Hafiz Ahmet [10], Abdullah [11] and Napolyon [12] grown in Turkey (The numbers in square brackets represent the cultivars [Cv.] for the equation). Lamina width, length and leaf area were measured to develop the model. The actual leaf area of the cultivars were measured by PLACOM Digital planimeter, and multiple regression analysis with Excel 7.0 computer package program was performed for the cultivars separately. The produced Leaf Area Prediction Model in the present study is LA=-22.45+2.59*W+4.76*L+0.36*Cv.- 0.23*L2 + 0.034*W* L2 - 0.002*Cv.* L2 (r2=0.9554) where LA is leaf area, W is leaf width, L is leaf length, Cv. is cultivar. In addition to model producing procedure, the model was validated using the residual values between predicted and measured leaf areas from new leaf samples of different cherry orchards. Coefficient of determination r2 values for the relationships between actual and predicted leaf areas of the tested cherry cultivars were found to be 0.9852, 0.9811, 0.989, 0.9856, 0.9894, 0.9841, 0.9794, 0.9962, 0.9909, 0.9759, 0.9867 and 0.9913 for Hüsenba [1], Lambert [2], 0900 Ziraat [3], Van [4], Bing [5], Bella di Pistoia [6], Stella [7], Early Burlat [8], Karakirtik [9], Hafiz Ahmet [10], Abdullah [11] and Napolyon [12], respectively.

361-367 Download
12
MULTIVARIATE ANALYSES IN CHICKPEA (CICER ARIETINUM L.)
ABDUL GHAFOOR, ZAHOOR AHMAD, ASIF JAVAID AND MUHAMMAD ASHRAF*

MULTIVARIATE ANALYSES IN CHICKPEA (CICER ARIETINUM L.)
ABSTRACT:
Thirty genotypes of chickpea were evaluated for 10 quantitative traits using multivariate techniques. The first three PCs with eigen values >1 contributed 83.38% of the variability amongst genotypes. Populations with high PC1 values were high yielding and characterized by high yield potential

369-376 Download
13
GENETIC DISSIMILARITIES IN COWPEA (VIGNA UNGUICULATA (L.) WALP.) FOR PROTEIN PEPTIDES AND THEIR SIGNIFICANCE FOR QUANTITATIVE TRAITS LOCI
MUHAMMAD SAJJAD IQBAL, ABDUL GHAFOOR*, AFSARI SHARIF QURESHI AND ZAHOOR AHMAD*

GENETIC DISSIMILARITIES IN COWPEA (VIGNA UNGUICULATA (L.) WALP.) FOR PROTEIN PEPTIDES AND THEIR SIGNIFICANCE FOR QUANTITATIVE TRAITS LOCI
ABSTRACT:
Cowpea (Vigna unguiculata (L.) Walp.), gemplasm comprising of diverse 138 accessions was evaluated for 23 physiological and agronomic characters during summer 2000 at NARC, Islamabad. The same material was analyzed for total seed protein using Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Out of 40 protein subunits, 31 were polymorphic and 9 were monomorphic. Out of polymorphic bands

377-386 Download
14
DETERMINING RELATIONSHIPS AMONG YIELD AND YIELD COMPONENTS USING CORRELATION AND PATH COEFFICIENT ANALYSES IN SUMMER SOWN COMMON VETCH (VICIA SATIVA L.) GENOTYPES
SADIK CAKMAKCI*, BILAL AYDINOGLU AND MEHMET KARACA

DETERMINING RELATIONSHIPS AMONG YIELD AND YIELD COMPONENTS USING CORRELATION AND PATH COEFFICIENT ANALYSES IN SUMMER SOWN COMMON VETCH (VICIA SATIVA L.) GENOTYPES
ABSTRACT:
Common vetch (Vicia sativa L.) is an important leguminous crop. Its breeding program can be improved by understanding the interrelationships among yield and yield components. Using 152 summer-sown genotypes originated from different sources, the present study was conducted in Mediterranean basin of Antalya, Turkey, with the aims to determine relationships among 13 agronomic characters and evaluate estimates of predicted direct and indirect responses to selection for characters using correlation and path coefficient analyses. Results based on the correlation analysis indicated that seed yield was correlated positively with biologic yield (0.810**), spring vigor (0.681**), number of seeds per plant (0.486**), harvest index (0.423**) and number of pods per plant (0.418**). However, when path coefficient analysis split the correlation coefficients into direct and indirect effects, the significant direct effects on the seed yield were biologic yield (0.795), harvest index (0.490) and number of seeds per plant (0.105). Biologic yield was correlated positively with seed yield (0.810**), spring vigor (0.777**), number of seeds per plant (0.462**), number of pods per plant (0.450**) and plant height (0.301**). There was negative direct effect of harvest index on biologic yield (-0.470). Overall results suggested that common vetch breeding studies should focus on biologic yield, harvest index, number of seeds per plant for seed yield, and seed yield, spring vigor and harvest index traits should be taken in account for biologic yield.

387-400 Download
15
NATURAL OCCURRENCE OF TOBACCO STREAK VIRUS IN COTTON IN PAKISTAN AND SCREENING FOR ITS RESISTANT SOURCES
WAQAR AHMED*, TAHIR BASIT BUTT, JAVED IHSAN AND ABDUL REHMAN

NATURAL OCCURRENCE OF TOBACCO STREAK VIRUS IN COTTON IN PAKISTAN AND SCREENING FOR ITS RESISTANT SOURCES
ABSTRACT:
During 1994, a cotton mosaic disease was observed on the leaves of cotton plants at several locations in cotton growing areas of the Punjab. Infected leaves were showing typical symptoms of mosaic. Twenty six different cotton varieties belonging to the Gossypium hirsutum group were screened against cotton mosaic under the natural infection conditions in different ecological zones. Among these, cotton varieties CIM-70, S-12, B-622, B-30, B-496, BH-4, BH-89, BH-94, BH-95, and Krishma showed resistance to mosaic but these were highly susceptible to cotton leaf curl virus (CLCuV). None of the cotton varieties tested was resistant to both CLCuV and Tobacco Streak Virus (TSV). Based on ELISA, TSV was detected in samples showing mosaic symptoms. TSV was readily graft transmissible but not transmissible by mechanical means. No evidence of its transmission through seeds or by thrips was obtained.

401-408 Download
16
SCREENING OF SUBSTRATES FOR MASS PRODUCTION OF BIOCONTROL AGENTS
SHAHNAZ DAWAR AND A. GHAFFAR

SCREENING OF SUBSTRATES FOR MASS PRODUCTION OF BIOCONTROL AGENTS
ABSTRACT:
Eleven different substrates viz., rice grain, sorghum grain, millet grain, cotton cake, mustard cake, wheat straw, rice straw, saw dust, sugarcane bagasse, sugarcane ash and wheat bran were used for the mass production of biocontrol agents viz., Paecilomyces lilacinus, Trichoderma harzianum, Gliocladium virens and Rhizobium meliloti. Rice grain, sorghum grain, millet grain and wheat bran were found suitable substrates for mass production of P. lilacinus and R. meliloti. Good growth of T. harzianum and G. virens was observed on sorghum grain followed by millet grain, rice grain, wheat bran, wheat straw, rice straw and sugar cane bagasse. Oil cakes, sugar cane ash and saw dust were found not suitable substrates for multiplication of biocontrol agents. The inoculum multiplied and stored in plastic bags remained viable for upto 360 days at 30°C.

409-414 Download
17
CHARACTERIZATION OF RHIZOBIA ISOLATED FROM SOME TREE LEGUMES GROWING AT THE KARACHI UNIVERSITY CAMPUS
RAIHA QADRI AND A. MAHMOOD

CHARACTERIZATION OF RHIZOBIA ISOLATED FROM SOME TREE LEGUMES GROWING AT THE KARACHI UNIVERSITY CAMPUS
ABSTRACT:
Rhizobial isolates from nodules of Albizia lebbeck (L.) Benth., and Samanea saman (Jacq.) Merr., were alkali-producing, slow growing and with monotrichous flagella, whereas, those from Pithecellobium dulce (Roxb.) Benth., and Dalbergia sissoo Roxb., were acid-producing and fast growers. Isolates from P. dulce showed amphitrichous flagella, while lophotrichous flagella were found in the isolates of D. sissoo. Rhizobial isolates from D. sissoo and P. dulce utilized all the 11 sugars used as carbon source, whereas isolates from A. lebbeck utilized 8, and those of S. saman utilized 7 out of 11 sugars. All isolates belonging to Mimosoideae were susceptible to Gentamycin, Neomycin and Tetracycline, but were resistant to Cephalexin, whereas reaction against other antibiotics was variable. Isolates of D. sissoo were resistant against Amoxicillin, Ampicillin, Cloxacillin, Erythromycin, Neomycin and Sulphamethoxazole Trimethoprim and susceptible against Gentamycin and Tetracycline.

415-421 Download
18
ISOLATION AND PARTIAL PURIFICATION OF ELICITOR MOLECULES FROM COLLETOTRICHUM LINDEMUTHIANUM
FATIMA BI* AND PAUL FINCH

ISOLATION AND PARTIAL PURIFICATION OF ELICITOR MOLECULES FROM COLLETOTRICHUM LINDEMUTHIANUM
ABSTRACT:
Colletotrichum lindemuthianum grown in glucose/neopeptone natural liquid medium showed that yield of crude 'High Molecular Weight Culture Filtrate Elicitor' (HMWCFE) obtained by simultaneous ultrafiltration and dialysis were little high 0.8 g/L in 6th day culture. A range of browning was observed in response to various elicitor preparations of each day culture of C.lindemuthianum. Maximum browning was produced by the elicitor preparation in 6th day culture. Total carbohydrate and protein contents were found to be 18.4 -33.4% and about 1.6-2.7% respectively. The presence of phosphate (4-12%) in high molecular weight culture filtrate elicitor is reported for the first time from C. lindemuthianum. Preliminary elution profile and apparent range of molecular weight of HMWCFE was >60 000 dalton determined by gel permeation chromatography.

423-429 Download
19
EFFECT OF ASCOCHYTA BLIGHT ON THE PRODUCTIVITY OF CHICKPEA
S.M. IQBAL, A. GHAFOOR, N. AYUB*, I. AHMAD AND A. BAKHSH

EFFECT OF ASCOCHYTA BLIGHT ON THE PRODUCTIVITY OF CHICKPEA
ABSTRACT:
Seven cultivars of chickpea viz., C-44, C-727, CM-72, Dasht, Parbat, NIFA-88 and Punjab-91 were tested to study the genotypic response to Ascochyta blight in terms of losses to grain yield and its components. Disease severity index of the cultivars ranged from 44 to 82%. Maximum disease at vegetative stage was recorded on C-727 followed by C-44 and Punjab-91. Minimum disease at vegetative stage was observed on Dasht. Pod infection that varied from 17 to 90% was highest on C-727 and least on Dasht. Minimum (2%) and maximum (42%) seed infection was respectively found in NIFA-88 and C-727. Comparison of data on number of pods per plant, seeds per plant

430-436 Download
20
EFFECT OF SOME TURKISH PROPOLIS ON THE PRODUCT QUANTITY OF AGARICUS BISPORUS (LANGE.) SING
PERIHAN GÜLER*, KADRIYE SORKUN** AND BEKIR SALIH***

EFFECT OF SOME TURKISH PROPOLIS ON THE PRODUCT QUANTITY OF AGARICUS BISPORUS (LANGE.) SING
ABSTRACT:
In this study, the effect of some Turkish propolis on the product quantity of cultivated mushroom Agaricus bisporus (Lange.) Sing. was determined. The samples of propolis were obtained from (Bursa and Erzurum) regions of Turkey. Propolis extracts were prepared as 0.5 EEP and 2.0 EEP and sprayed on compost, compost + casing soil and compost + casing soil + 1 flush of Agaricus bisporus at different times of growth period under controlled laboratory conditions Propolis showed stimulatory effects on the developmental stages and some parameters of the yield. An early yield of mushrooms, rapid growing and increase of total weight of harvested basidiocarps were observed as compared to control without propolis in which primordium and basidiocarp formations showed great reduction. Chemical analysis of all the harvested mushroom that were cultivated on the product conditions with propolis were made by gas chromotography (GC)-mass spectrometry (MS).

437-445 Download
21
HYALOTIASTRUM GEN. NOV., AN ADDITION TO COELOMYCETES FROM PAKISTAN
SYED QAISER ABBAS, B.C. SUTTON* A.GHAFFAR** AND ALIA ABBAS

HYALOTIASTRUM GEN. NOV., AN ADDITION TO COELOMYCETES FROM PAKISTAN
ABSTRACT:
Hyalotlastrum gen. nov., et H. salvadorae sp. nov., belonging to Coelomycetes are illustrated, described and compared with related taxa.

447-454 Download
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